Journal: Cell

Article Title: Inflammation Improves Glucose Homeostasis Through IKKβ-XBP1s Interaction

doi: 10.1016/j.cell.2016.10.015

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-phospho-XBP1s Thr48 , Covance , Raised against CRAAGSEASGT(p)PQARKRQR.

Techniques: Recombinant, Plasmid Preparation, Expressing, Sequencing, Software

Journal: Frontiers in Cell and Developmental Biology

Article Title: Obesity impacts placental function through activation of p-IRE1a-XBP1s signaling

doi: 10.3389/fcell.2023.1023327

Figure Lengend Snippet: Primers used for the QPCR.

Article Snippet: Following antibodies were used in this study: rabbit anti-p-IRE1α antibody (Thermofisher Scientific, Cat #PA1-16927, 1:2,000); rabbit anti- XBP1s antibody (ProteinTech, Cat #24868-1-AP, 1:2,000); Rabbit anti-p-Perk antibody (Thermofisher Scientific, Cat #PA5-37773, 1:2,000); and rabbit anti-Bip antibody (Cell Signaling Technology, Cat #CST-31776, 1:2,000).

Techniques:

Journal: Frontiers in Cell and Developmental Biology

Article Title: Obesity impacts placental function through activation of p-IRE1a-XBP1s signaling

doi: 10.3389/fcell.2023.1023327

Figure Lengend Snippet: Obesity activates XBP1 splicing in the placenta. XBP1s specific antibody was used to label activated XBP1s. Compared to the non-obese placenta (A) , n = 10), the obese placenta exhibited increased XBP1s (B) , n = 12). Elevated XBP1s was visualized by PCR with a primer pair flanking splicing regions followed by electrophoresis (C) (unspliced, 256 bp; spliced XBP1, 230 bp) . Quantified with ImageJ on the immunocytochemically labeled sections (D) or XBP1s PCR gel images (E) showed increased XBP1s in the obese placenta. Furthermore, Reverse Transcription (RT)-QPCR demonstrated increase of XBP1s in the obese placenta (F) . Non-parametric Mann-Whitney U -test was used to compare the XBP1s between the non-obese and obese placenta. Values are mean ± SEM. N1-N12: non-obese samples; O1-O12: obese samples.

Article Snippet: Following antibodies were used in this study: rabbit anti-p-IRE1α antibody (Thermofisher Scientific, Cat #PA1-16927, 1:2,000); rabbit anti- XBP1s antibody (ProteinTech, Cat #24868-1-AP, 1:2,000); Rabbit anti-p-Perk antibody (Thermofisher Scientific, Cat #PA5-37773, 1:2,000); and rabbit anti-Bip antibody (Cell Signaling Technology, Cat #CST-31776, 1:2,000).

Techniques: Electrophoresis, Labeling, Reverse Transcription, Quantitative RT-PCR, MANN-WHITNEY

Journal: Cell reports

Article Title: MEF2A suppresses stress responses that trigger DDX41-dependent IFN production

doi: 10.1016/j.celrep.2023.112805

Figure Lengend Snippet:

Article Snippet: Rabbit anti XBP-1s (D2CF1F) , Cell Signaling Technology , RRID:AB_2687943.

Techniques: Virus, Recombinant, cDNA Synthesis, Bicinchoninic Acid Protein Assay, Enzyme-linked Immunosorbent Assay, RNA Sequencing, Negative Control, Software